Radon Dynamics and Effective Dose Estimation in a Touristic Volcanic Cave: La Cueva del Viento, Tenerife (Canary Islands, Spain).

La Cueva del Viento is a volcanic lava tube located in Tenerife Island (Canary Islands, Spain). Its touristic section, 180 m long, receives more than 28,200 visitants each year. According to the European and Spanish legislation, a radon monitoring program is required to minimize the radon exposition of workers, tourists, and cavers. In this work, we studied the radon concentration dynamics in the touristic section of the cave for ca. 1 year, using both passive and active radon detectors. Pluviometry and external air temperature played an important role in the seasonal and daily variations of indoor radon concentrations. Daily fluctuations during the dry season were analyzed using time series (Box-Jenkins methodology) and frequency analysis (Fourier and Wavelet transforms) methods. The experimental radon time-series was well-fitted using a seasonal autoregressive integrated moving average model: Seasonal Auto-Regressive Integrated Moving Average (2,0,1) (2,1,0)24, and its value, in a short-time window (ca. 1 week) was conveniently forecasted. Finally, this work revealed that the annual effective doses received, during the observation period (1 year), by the touristic guides and visitors was ca. 2 mSv/yr and 4 µSv/hr, respectively. We concluded that the touristic exploitation of La Cueva del Viento is safe for both tourists and guides. However, based on our results, La Cueva del Viento had to be classified as a "Monitoring zone" and a regular monitoring program should be implemented.

[Suicidal attempt and suicidal ideation during the COVID-19 pandemic compared to previous years]. / Intentos e ideas de suicidio durante la pandemia por COVID-19 en comparación con los años previos.

INTRODUCTION: Since the COVID-19 pandemic began, warnings have been made about the impact on the mental health of the population and as occurred in previous pandemics, an increase in suicide rates had been anticipated. To date, studies differ with regard to whether suicide rates are being affected during the COVID pandemic. MATERIAL AND METHODS: All individuals registered for suicidal ideation or suicidal attempt in the Suicide Risk Code (CRS) from the city of Barcelona from January 1, 2018 to June 30, 2021 have been included. A bivariate analysis has been carried out between the 2018-2019 period and the pandemic period. The percentage increase in the monthly incidence of CRS cases has been calculated, using the average monthly incidence for the 2018-2019 period as a reference. RESULTS: A total of 3388 consultations for suicide ideation or suicidal attempt were registered. There has been an increase of 43.20% in the monthly incidence of suicidal ideation and suicidal attempts during the pandemic compared to the 2018-2019 period, reaching a maximum increase of 573.8% in young people under 18 years in the month of May 2021. CONCLUSIONS: During the COVID-19 pandemic period, consultations for suicidal ideation and suicidal attempts have increased compared to the previous 2 years in the city of Barcelona. It should be noted this growth especially in young people under 18 years.

Síntomas neuropsiquiátricos como factor de confusión en la detección de la demencia / No disponible

OBJETIVO: Valorar si los síntomas neuropsiquiátricos interfieren en la detección de deterioro cognitivo por los médicos de familia en atención primaria, así como describir cuáles generan más confusión. DISEÑO: Estudio observacional y descriptivo. Emplazamiento: Equipo de psiquiatría de intervención en domicilio en colaboración con la red de atención primaria de Barcelona. PARTICIPANTES: Un total de 104 pacientes mayores de 65 años derivados desde atención primaria por sus médicos de familia solicitando valoración psiquiátrica en el domicilio por sospecha de enfermedad mental. Mediciones principales: Todos los casos recibieron un diagnóstico según criterios DSM-IV-TR. Se incluyeron en el estudio el Mini Mental State Examination (MMSE), el Inventario Neuropsiquiátrico de Cummings, la escala de Gravedad de Enfermedad Psiquiátrica, la escala de Evaluación de la Actividad Global, la escala de Impresión Clínica Global y el Cuestionario de Evaluación de la Discapacidad de la Organización Mundial de la Salud. RESULTADOS: El 55,8% de los pacientes derivados desde atención primaria tenían el MMSE alterado. Los síntomas neuropsiquiátricos más frecuentemente asociados a la sospecha de deterioro cognitivo fueron los delirios, las alucinaciones, la agitación, la desinhibición, la irritabilidad y la conducta motora sin finalidad. CONCLUSIONES: Cuando se detecten síntomas psiquiátricos propios de trastorno mental severo (TMS) en individuos de edad avanzada sin antecedentes de TMS hay que sospechar un deterioro cognitivo y se debería administrar una prueba de cribado

OBJECTIVE: The aim of the study was to evaluate whether the neuropsychiatric symptoms interfere with cognitive impairment detection in primary care and to describe which of them generate more confusion. DESIGN: Descriptive and observational study. LOCATION: Mobile psychiatric unit in collaboration with primary healthcare centers in Barcelona. PARTICIPANTS: A total of 104 patients over 65 years referred to mobile psychiatric unit from primary healthcare clinicians suspecting mental disease. MAIN MEASUREMENTS: All patients received a DSM-IV-TR diagnosis. We included in the study the Mini Mental State Examination (MMSE), Neuropsichiatric Inventory, Severe Psychiatric Illness scale, Global Assessment of Functioning, Clinical Global Impression and Word Health Organisation Dissability Assessment Schedule. RESULTS: 55.8% of patients referred from primary care had altered MMSE score. Neuropsychiatric symptoms more frequently associated with suspected cognitive impairment were delusions, hallucinations, agitation, disinhibition, irritability and purposeless motor behavior. CONCLUSIONS: When psychiatric symptoms of Severe Mental Disorder (SMD) are detected in elderly individuals with no history of SMD, cognitive impairment should be suspected and a screening test be done

Síntomas neuropsiquiátricos como factor de confusión en la detección de la demencia.

OBJECTIVE: The aim of the study was to evaluate whether the neuropsychiatric symptoms interfere with cognitive impairment detection in primary care and to describe which of them generate more confusion. DESIGN: Descriptive and observational study. LOCATION: Mobile psychiatric unit in collaboration with primary healthcare centers in Barcelona. PARTICIPANTS: A total of 104 patients over 65years referred to mobile psychiatric unit from primary healthcare clinicians suspecting mental disease. MAIN MEASUREMENTS: All patients received a DSM-IV-TR diagnosis. We included in the study the Mini Mental State Examination (MMSE), Neuropsichiatric Inventory, Severe Psychiatric Illness scale, Global Assessment of Functioning, Clinical Global Impression and Word Health Organisation Dissability Assessment Schedule. RESULTS: 55.8% of patients referred from primary care had altered MMSE score. Neuropsychiatric symptoms more frequently associated with suspected cognitive impairment were delusions, hallucinations, agitation, disinhibition, irritability and purposeless motor behavior. CONCLUSIONS: When psychiatric symptoms of Severe Mental Disorder (SMD) are detected in elderly individuals with no history of SMD, cognitive impairment should be suspected and a screening test be done.

Study of fingerprints in Argentina population for application in personal identification.

The fingerprints of the Buenos Aires and Chubut provinces in Argentina have been studied, with the aim of knowing and quantifying the variability of these features, which are used in the identification process. The data studied for this research was obtained from 330 individuals, of both sexes, from two Argentinian population samples (170 individuals from Buenos Aires and 160 from Chubut), which amounts to a total of 3300 fingerprints. The different types of minutiae were located, identified, and visually quantified in four areas on the fingerprint. Two perpendicular axes were drawn whose intersection was located in the center of fingerprint. In addition, a circle was defined on these quadrants whose radius cut fifteen ridges. This method divides the fingerprints into four quadrants with two sectors apiece. The results obtained for both populations were compared statistically with those published previously for an Argentinian population sample, which had been collected using the same methodology. Therefore, Argentina becomes the country with the most information in this matter. For both populations, the highest frequencies were of ridge endings, followed by bifurcations and convergences. In this study of minutiae, statistically significant differences were found between the area of the fingerprint (inside and outside the circle), males and females, and types of main pattern for both samples. However, although the results show common patterns in the distribution of minutiae, there are also significant differences between populations. This reveals a significant ancestral and frequency effect of the minutiae, which would indicate that the minutiae are more genetically dependent than has been suspected so far. Furthermore, the non-equiprobability found for the frequency of the types of minutiae indicates that the weight provided by these characteristics is not the same when applied in identification processes, whether used quantitatively (numerical standard) or qualitatively (holistic method).

Home treatment in preventing hospital admission for moderate and severe mentally ill people.

A Home Treatment team was compared with a Psychiatric Emergency Department (PED) on preventing hospitalization among two cohorts of 448 patients matched by diagnosis, sex, and age. The logistic regression showed that HT people were 4.6 times less likely to be admitted than those from PED.

A comparative study of topological and sex differences in fingerprint ridge density in Argentinian and Spanish population samples.

Although several studies have recently assessed sex differences in fingerprint ridge density and its variability in human populations from different origins, such a study has not been carried out yet in the Amerindian population. The goal of this study was to determine the topological and sexual differences in fingerprint ridge density (RD) in native subjects from two samples of northwestern Argentina (Jujuy province) living at different altitudes. The results were compared with those obtained from a Spanish population sample. The study was based on data from all 10 fingerprints of 393 adult Argentinian men and women, 193 from the Puna-Quebrada region (more than 2500 m above sea level) and 200 from Ramal (500 m above sea level). Ridge density was assessed for three different areas (radial, ulnar and proximal) for all 10 fingers of each subject. In both samples, significant differences between areas were obtained, so radial RD > ulnar RD > proximal RD. No significant differences were found between samples in males, while females from both samples significantly differed in the radial and proximal areas. Females have higher RD, so narrower ridges, than men, in all areas and all fingers. Application of Bayes' theorem allowed us to obtain a ridge density threshold for discrimination of sexes in Argentinian samples and the threshold for discrimination of populations between Argentinian and Spanish samples. These results can be useful for forensic use.

Molecular characterisation of the amino- and carboxyl-domains in different Glu-A1x alleles of Triticum urartu Thum. ex Gandil.

The wild diploid wheat (Triticum urartu Thum. ex Gandil.) is a potential gene source for wheat breeding, as this species has been identified as the A-genome donor in polyploid wheats. One important wheat breeding trait is bread-making quality, which is associated in bread wheat (T. aestivum ssp. aestivum L. em. Thell.) with the high-molecular-weight glutenin subunits. In T. urartu, these proteins are encoded by the Glu-A1x and Glu-A1Ay genes at the Glu-A (u) 1 locus. The Glu-A1x genes of 12 Glu-A (u) 1 allelic variants previously detected in this species were analysed using PCR amplification and sequencing. Data showed wide diversity for the Glu-A1x alleles in T. urartu, which also showed clear differences to the bread wheat alleles. This variation could enlarge the high-quality genetic pool of modern wheat and be used to diversify the bread-making quality in durum (T. turgidum ssp. durum Desf. em. Husn.) and common wheat.

Waxy genes from spelt wheat: new alleles for modern wheat breeding and new phylogenetic inferences about the origin of this species.

BACKGROUND AND AIMS: Waxy proteins are responsible for amylose synthesis in wheat seeds, being encoded by three waxy genes (Wx-A1, Wx-B1 and Wx-D1) in hexaploid wheat. In addition to their role in starch quality, waxy loci have been used to study the phylogeny of wheat. The origin of European spelt (Triticum aestivum ssp. spelta) is not clear. This study compared waxy gene sequences of a Spanish spelt collection with their homologous genes in emmer (T. turgidum ssp. dicoccum), durum (T. turgidum ssp. durum) and common wheat (T. aestivum ssp. aestivum), together with other Asian and European spelt that could be used to determine the origin of European spelt. METHODS: waxy genes were amplified and sequenced. Geneious Pro software, DNAsp and MEGA5 were used for sequence, nucleotide diversity and phylogenetic analysis, respectively. KEY RESULTS: Three, four and three new alleles were described for the Wx-A1, Wx-B1 and Wx-D1 loci, respectively. Spelt accessions were classified into two groups based on the variation in Wx-B1, which suggests that there were two different origins for the emmer wheat that has been found to be part of the spelt genetic make-up. One of these groups was only detected in Iberian material. No differences were found between the rest of the European spelt and the Asiatic spelt, which suggested that the Iberian material had a different origin from the other spelt sources. CONCLUSIONS: The results suggested that the waxy gene variability present in wheat is undervalued. The evaluation of this variability has permitted the detection of ten new waxy alleles that could affect starch quality and thus could be used in modern wheat breeding. In addition, two different classes of Wx-B1 were detected that could be used for evaluating the phylogenetic relationships and the origins of different types of wheat.

Are there population differences in minutiae frequencies? A comparative study of two Argentinian population samples and one Spanish sample.

In recent years, both scientific and judicial sources have highlighted the need for more knowledge about minutiae variability, in order to improve their statistical application to fingerprint identification. In line with this trend toward improving our knowledge of this subject, the aim of the present study was to calculate the frequency with which 20 types of minutiae appeared in 2780 fingerprint impressions obtained from 278 individuals from two Argentinian population samples (100 individuals from Ramal and 178 from Puna-Quebrada). The different types of minutiae were located, identified, and quantified visually in two areas on the fingerprint, the inside and outside of a circle, the radius of which cut fifteen ridges perpendicularly, starting from the center cut of the axes defining the sectors. The non-equiprobability found in both population samples for the different minutiae types studied demonstrated that the evidential weight provided by these characteristics is not the same when applied in identification processes, whether used quantitatively (numerical standard) or qualitatively (holistic method). The results obtained for both populations were compared statistically with those published previously for a Spanish population sample, which had been collected using the same methodology. This comparison has enabled us to demonstrate, for the first time, the existence of significant differences between populations in minutiae frequencies, independently from the main pattern type.

Microsatellite development for the relictual conifer Araucaria araucana (Araucariaceae) using next-generation sequencing.

PREMISE OF THE STUDY: In this study, the 454 GS-FLX genome sequence system was used for the identification and characterization of microsatellites in Araucaria araucana, one of the most important and endangered species of Chilean and Argentinean native forests. METHODS AND RESULTS: A total of 35876 reads were identified, 96% of which were within the size range selected for further analysis. Of these, 1563 contained a microsatellite insert suitable for primer design. Ten simple sequence repeat (SSR) markers provided easily interpretable patterns and were used to evaluate the genetic diversity in four populations of the species. The 10 microsatellites showed high polymorphism levels, with a total of 99 alleles and 32 private alleles. The observed heterozygosity was high and ranged from 0.513 to 0.723. CONCLUSIONS: The primers presented in this study display high genetic diversity and may provide useful information for the design of conservation strategies in Araucaria araucana.

Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique.

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geographic origin.